CALML6 Polyclonal antibody

CALML6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22274-1-AP

Synonyms

CAGLP, Calglandulin like protein, CALGP, CALML6, calmodulin like 6, Calmodulin like protein 6

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Validation Data Gallery

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  WB analysis of human heart using 22274-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 22274-1-AP (CALML6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 22274-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 22274-1-AP (CALML6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 22274-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22274-1-AP (CALML6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22274-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22274-1-AP (CALML6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 22274-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22274-1-AP (CALML6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 22274-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22274-1-AP (CALML6 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human heart tissue, pig heart tissue
• Positive IHC detected in: human heart tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

22274-1-AP targets CALML6 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calmodulin-like 6
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: NM_138705
• Gene Symbol: CALML6
• Gene ID (NCBI): 163688
• RRID: AB_11182942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CALML6, also named as CAGLP, CALGP , belongs to the calmodulin family and Calglandulin subfamily. It contains 4 EF-hand domains. The antibody is specific to CALML6.

Publications

Species	Application	Title
human	IHC	Technol Cancer Res Treat; A Pyroptosis-Related Gene Signature Predicts Prognosis and Tumor Immune Microenvironment in Colorectal Cancer; Authors - Linjing Li
human	IHC	Gene; CALML6 as a potential diagnostic marker for thyroid cancer promotes thyroid cancer cell proliferation via modulating the immune microenvironment; Authors - Caixia Cheng