CAMK2B Recombinant monoclonal antibody, PBS Only (Detector)

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• CAMK2B Recombinant monoclonal antibody, PBS Only (Detector) (86858-1-PBS)

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  WB analysis of U-251 using 86858-1-RR (same clone as 86858-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86858-1-RR (CAMK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86858-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02919-1

  The mean CAMK2B concentration was determined to be 130.09 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 669.36 ng/mL in HEK-293T cell extract based on a 1.20 mg/mL extract load, 436.22 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 332.36 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 124.24 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02919-1

  Sandwich ELISA standard curve of MP02919-1, Human CAMK2B Recombinant Matched Antibody Pair - PBS only. 86858-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2119. 86858-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 86858-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86858-1-RR against Human CAMK2B were performed. The affinity constant is below 1 pM.

CAMK2B Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 86858-1-PBS

Clone No.251925D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2

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Product Information

86858-1-PBS targets CAMK2B as part of a matched antibody pair:

MP02919-1: 86858-2-PBS capture and 86858-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2119

  Product name: Recombinant human CAMK2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC019070

  Sequence: MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFS

• Full Name: calcium/calmodulin-dependent protein kinase II beta
• Calculated Molecular Weight: 503 aa, 56 kDa
• Observed Molecular Weight: 50-70 kDa
• GenBank Accession Number: BC019070
• Gene Symbol: CaMKII beta
• Gene ID (NCBI): 816
• RRID: AB_3745169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13554
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.