CAMKV Polyclonal antibody, PBS Only

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• CAMKV Polyclonal antibody, PBS Only (14788-1-PBS)

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  WB analysis of mouse brain using 14788-1-AP (same clone as 14788-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14788-1-AP (same clone as 14788-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 14788-1-AP (same clone as 14788-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 14788-1-AP (same clone as 14788-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14788-1-AP (CAMKV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14788-1-AP (same clone as 14788-1-PBS)

  IP result of anti-CAMKV (IP:14788-1-AP, 4ug; Detection:14788-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 14788-1-AP (same clone as 14788-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14788-1-AP (CAMKV antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14788-1-PBS in a different storage buffer formulation.

CAMKV Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA

Cat No. 14788-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, Indirect ELISA

Synonyms

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Product Information

14788-1-PBS targets CAMKV in WB, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6438

  Product name: Recombinant human CAMKV protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-285 aa of BC000497

  Sequence: MPFGCVTLGDKKNYNQPSEVTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDGRKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSERDTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEPCGTPEYLAPEVVGRQRYGRPVDCWAIGVIMYILLSGNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRITAEEAISHEW

• Full Name: CaM kinase-like vesicle-associated
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-75 kDa
• GenBank Accession Number: BC000497
• Gene Symbol: CAMKV
• Gene ID (NCBI): 79012
• RRID: AB_2068552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCB2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).