CAP1 Monoclonal antibody, PBS Only

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  WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

  WB result of CAP1 antibody (68207-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 68207-1-Ig (same clone as 68207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68207-1-PBS

Clone No.2H3A10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

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Product Information

68207-1-PBS targets CAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8876

  Product name: Recombinant human CAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 125-475 aa of BC013963

  Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

• Full Name: CAP, adenylate cyclase-associated protein 1 (yeast)
• Calculated Molecular Weight: 475 aa, 52 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC013963
• Gene Symbol: CAP1
• Gene ID (NCBI): 10487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q01518
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.