Calpain 1 Polyclonal antibody

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  WB analysis of HeLa using 10538-1-AP

  WB result of Calpain 1 antibody (10538-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 1 transfected HeLa cells.

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  WB analysis using 10538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10538-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10538-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 10538-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10538-1-AP

  IP result of anti-Calpain 1 (IP:10538-1-AP, 4ug; Detection:10538-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human urothelial carcinoma using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10538-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Calpain 1 antibody (10538-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HeLa using 10538-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Calpain 1 Polyclonal antibody (10538-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Calpain 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10538-1-AP

Publications(37)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CAPN1, Calpain1, Calpain mu-type, Calpain mu type, Calpain 1 large subunit

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Tested Applications

• Positive WB detected in: HeLa cells, A2780 cells, A431 cells, mouse testis tissue, HepG2 cells, PC-3 cells, K-562 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human urothelial carcinoma tissue, human heart tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 35 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

10538-1-AP targets Calpain 1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 1 fusion protein Ag0810
• Full Name: calpain 1, (mu/I) large subunit
• Calculated Molecular Weight: 704 aa, 82 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC008751
• Gene Symbol: CAPN1
• Gene ID (NCBI): 823
• RRID: AB_10858627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07384
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	J Ginseng Res; Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway; Authors - Fang Zhao
mouse	WB	Pharmacol Res; TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.; Authors - Wen Tian
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang
mouse	WB	Int Immunopharmacol; The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice; Authors - Mengqing Liu
human	WB	J Biol Chem; Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.; Authors - Hui-Yan Lei
human,mouse	WB	Biochim Biophys Acta Mol Cell Res; Elastic titin properties and protein quality control in the aging heart.; Authors - Senem Salcan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-09-2024)

Obtained a strong western blot signal in NRVCMs

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs

FH

Brice-Emmanuel (Verified Customer) (09-11-2023)

Works perfectly, I obtained a single strip of the desired size using the 1/5000 dilution in NFDM 5%.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Mouse Heart Tissue

Calpain 1 Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:10538-1-AP)

FH

James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK cell overexpression

FH

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart