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  • KD/KO Validated

Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10538-1-AP


Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

Tested Applications

Positive WB detected inA431 cells, PC-3 cells, mouse testis tissue, Jurkat cells, HeLa cells, K-562 cells, A2780 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, human heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®650 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 1 fusion protein Ag0810
Full Name calpain 1, (mu/I) large subunit
Calculated molecular weight 704 aa, 82 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC008751
Gene symbol CAPN1
Gene ID (NCBI) 823
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Product Specific Protocols
WB protocol for Calpain 1 antibody 10538-1-APDownload protocol
IHC protocol for Calpain 1 antibody 10538-1-APDownload protocol
IF protocol for Calpain 1 antibody 10538-1-APDownload protocol
IP protocol for Calpain 1 antibody 10538-1-APDownload protocol
FC protocol for Calpain 1 antibody 10538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.

Authors - Xueyang Deng

J Biol Chem

Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.

Authors - Hui-Yan Lei

Physiol Rep

The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.

Authors - Takaya Kotani

Exp Ther Med

Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.

Authors - Pingheng Zhang

Ann Anat

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Authors - Shuchao Wang

Anticancer Res

Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.

Authors - Urszula Ciesielska


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK cell overexpression

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart