A2780 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP(Calpain 1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP(Calpain 1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP(Calpain 1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP(Calpain 1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP( Calpain 1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP( Calpain 1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-Calpain 1 (IP:10538-1-AP, 4ug; Detection:10538-1-AP 1:1000) with HeLa cells lysate 1200ug.

Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HeLa cells were stained with 0.2ug Calpain 1 antibody (10538-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.