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Calpain 1 Polyclonal antibody
Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10538-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse testis tissue, A2780 cells, A431 cells, HepG2 cells, PC-3 cells, K-562 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human urothelial carcinoma tissue, human heart tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calpain 1 fusion protein Ag0810|
|Full Name||calpain 1, (mu/I) large subunit|
|Calculated molecular weight||704 aa, 82 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC008751|
|Gene ID (NCBI)||823|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IHC protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IF protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IP protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.
TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.
J Biol Chem
Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.
Biochim Biophys Acta Mol Cell Res
Elastic titin properties and protein quality control in the aging heart.
Biochim Biophys Acta Mol Cell Biol Lipids
Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.
J Cell Mol Med
Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Brice-Emmanuel (Verified Customer) (09-11-2023)
Works perfectly, I obtained a single strip of the desired size using the 1/5000 dilution in NFDM 5%.
James (Verified Customer) (03-31-2021)
Antibody detected endogenous and overexpressed Calpain-1 in HEK cells
Wei (Verified Customer) (03-12-2020)
Single, strong western blot band at predicted size.