Tested Applications
| Positive WB detected in | HeLa cells, A2780 cells, A431 cells, mouse testis tissue, HepG2 cells, PC-3 cells, K-562 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human urothelial carcinoma tissue, human heart tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10538-1-AP targets Calpain 1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0810 Product name: Recombinant human CAPN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 413-714 aa of BC008751 Sequence: RESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFNALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA Predict reactive species |
| Full Name | calpain 1, (mu/I) large subunit |
| Calculated Molecular Weight | 704 aa, 82 kDa |
| Observed Molecular Weight | 75 kDa, 90 kDa |
| GenBank Accession Number | BC008751 |
| Gene Symbol | CAPN1 |
| Gene ID (NCBI) | 823 |
| RRID | AB_10858627 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07384 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calpain 1 is a calcium-dependent cysteine protease belonging to the calpain family. It functions as a heterodimer consisting of a catalytic large subunit of approximately 80 kDa and a regulatory small subunit of approximately 28 kDa, forming a holoenzyme of about 110 kDa; however, the large subunit is typically detected at 75-80 kDa by Western blotting. This protease plays critical roles in various physiological processes, including cytoskeletal remodeling, signal transduction, cell migration, apoptosis, and long-term potentiation, by executing limited proteolysis of substrate proteins (e.g., membrane proteins, kinases, and cytoskeletal proteins) to modulate their functions rather than to degrade them completely. Dysregulation of calpain 1 activity is closely associated with multiple pathological conditions, such as muscular atrophy, neurodegenerative diseases and ischemia-reperfusion injury.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
| IF protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
| IHC protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
| IP protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
| WB protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Calpain 1 (catalog no. 10538-1-AP) has 61 citations across journals including Nature Communications, Advanced Science, EMBO Molecular Medicine, Journal of Biological Chemistry, Pharmacological Research, Cell Death & Disease, Molecular Medicine, and Life Sciences. Research fields span cardiovascular disease (pulmonary hypertension, aortic dissection, cardiac hypertrophy), neurology (PTSD, depression, epilepsy), oncology (glioma, lung cancer, breast cancer), renal fibrosis, ferroptosis, autophagy, inflammation, virology, and food science.
| Species | Application | Title |
|---|---|---|
Nat Commun Loss-of-function variants in the CAPN1 activator CD99L2 cause X-linked spastic ataxia. | ||
J Nanobiotechnology Single-atom Ca nanozyme induces glioma death through Ca(2+)-overload-enhanced catalytic tumor nanotherapy, ferroptosis and synergistic remodeling of the immune microenvironment. | ||
Adv Sci (Weinh) Calpain-2-Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection | ||
Pharmacol Res TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy. | ||
Pharmacol Res Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. |
Reviews
What customers say
All four reviews are 5-star ratings from Western Blot applications. Users report strong, single bands at the expected molecular weight across multiple sample types including mouse heart tissue, NRVCMs, and HEK cells. The antibody was validated at dilutions of 1:1000 and 1:5000, with one user noting it successfully detected both endogenous and overexpressed Calpain-1. Overall, reviewers describe consistent, reliable performance with clean results and no mention of background or non-specific binding issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marco (Verified Customer) (07-09-2024) | Obtained a strong western blot signal in NRVCMs
|
Brice-Emmanuel (Verified Customer) (09-11-2023) | Works perfectly, I obtained a single strip of the desired size using the 1/5000 dilution in NFDM 5%.
 |
James (Verified Customer) (03-31-2021) | Antibody detected endogenous and overexpressed Calpain-1 in HEK cells
|
Wei (Verified Customer) (03-12-2020) | Single, strong western blot band at predicted size.
|
