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Calpain 1 Polyclonal antibody
Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 10538-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, PC-3 cells, mouse testis tissue, Jurkat cells, HeLa cells, K-562 cells, A2780 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human urothelial carcinoma tissue, human heart tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calpain 1 fusion protein Ag0810|
|Full Name||calpain 1, (mu/I) large subunit|
|Calculated molecular weight||704 aa, 82 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC008751|
|Gene ID (NCBI)||823|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IHC protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IF protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IP protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|FC protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.
J Biol Chem
Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.
The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.
Exp Ther Med
Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.
Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (03-31-2021)
Antibody detected endogenous and overexpressed Calpain-1 in HEK cells
Wei (Verified Customer) (03-12-2020)
Single, strong western blot band at predicted size.