- Featured Product
- KD/KO Validated
Calpain 1 Polyclonal antibody
Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10538-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, PC-3 cells, mouse testis tissue, Jurkat cells, HeLa cells, K-562 cells, A2780 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human urothelial carcinoma tissue, human heart tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 18 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Product Information
10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Calpain 1 fusion protein Ag0810 |
Full Name | calpain 1, (mu/I) large subunit |
Calculated molecular weight | 704 aa, 82 kDa |
Observed molecular weight | 75 kDa |
GenBank accession number | BC008751 |
Gene symbol | CAPN1 |
Gene ID (NCBI) | 823 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
IHC protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
IF protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
IP protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
FC protocol for Calpain 1 antibody 10538-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions. | ||
Physiol Rep The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle. | ||
Exp Ther Med Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling. | ||
Ann Anat Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons. | ||
Anticancer Res Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH James (Verified Customer) (03-31-2021) | Antibody detected endogenous and overexpressed Calpain-1 in HEK cells
|
FH Wei (Verified Customer) (03-12-2020) | Single, strong western blot band at predicted size.
|