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  • KD/KO Validated

Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10538-1-AP


Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

Tested Applications

Positive WB detected inHeLa cells, A2780 cells, A431 cells, mouse testis tissue, HepG2 cells, PC-3 cells, K-562 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, human heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10538-1-AP targets Calpain 1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 1 fusion protein Ag0810
Full Name calpain 1, (mu/I) large subunit
Calculated Molecular Weight 704 aa, 82 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC008751
Gene Symbol CAPN1
Gene ID (NCBI) 823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for Calpain 1 antibody 10538-1-APDownload protocol
IHC protocol for Calpain 1 antibody 10538-1-APDownload protocol
IF protocol for Calpain 1 antibody 10538-1-APDownload protocol
IP protocol for Calpain 1 antibody 10538-1-APDownload protocol
FC protocol for Calpain 1 antibody 10538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Ginseng Res

Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway

Authors - Fang Zhao

Pharmacol Res

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

Authors - Wen Tian

Pharmacol Res

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Authors - Juan Wang

Int Immunopharmacol

The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice

Authors - Mengqing Liu

J Biol Chem

Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.

Authors - Hui-Yan Lei

Biochim Biophys Acta Mol Cell Res

Elastic titin properties and protein quality control in the aging heart.

Authors - Senem Salcan


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Marco (Verified Customer) (07-09-2024)

Obtained a strong western blot signal in NRVCMs

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NRVCMs

Brice-Emmanuel (Verified Customer) (09-11-2023)

Works perfectly, I obtained a single strip of the desired size using the 1/5000 dilution in NFDM 5%.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: Mouse Heart Tissue
Calpain 1 Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:10538-1-AP)

James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK cell overexpression

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart