Calpain 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (2)

Cat no : 10538-1-AP

Synonyms

Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

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Validation Data Gallery

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  WB analysis of HeLa using 10538-1-AP

  WB result of Calpain 1 antibody (10538-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 1 transfected HeLa cells.

• 

  WB analysis of A431 using 10538-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10538-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10538-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10538-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10538-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10538-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10538-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10538-1-AP

  IP Result of anti-Calpain 1 (IP:10538-1-AP, 4ug; Detection:10538-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human urothelial carcinoma using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10538-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10538-1-AP

  Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 10538-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Calpain 1 antibody (10538-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A431 cells, PC-3 cells, mouse testis tissue, Jurkat cells, HeLa cells, K-562 cells, A2780 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human urothelial carcinoma tissue, human heart tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®650 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 1 fusion protein Ag0810
• Full Name: calpain 1, (mu/I) large subunit
• Calculated molecular weight: 704 aa, 82 kDa
• Observed molecular weight: 75 kDa
• GenBank accession number: BC008751
• Gene symbol: CAPN1
• Gene ID (NCBI): 823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Death Dis; Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.; Authors - Xueyang Deng
human	WB	J Biol Chem; Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.; Authors - Hui-Yan Lei
rat	WB	Physiol Rep; The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.; Authors - Takaya Kotani
human		Exp Ther Med; Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.; Authors - Pingheng Zhang
rat	WB,IF	Ann Anat; Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.; Authors - Shuchao Wang
human	WB	Anticancer Res; Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.; Authors - Urszula Ciesielska

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK cell overexpression

FH

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart