Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 10538-1-AP

Synonyms

Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL



Tested Applications

Positive WB detected inA431 cells, A2780 cells, HeLa cells, Jurkat cells, K-562 cells, mouse testis tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, human heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 1 fusion protein Ag0810
Full Name calpain 1, (mu/I) large subunit
Calculated molecular weight 704 aa, 82 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC008751
Gene symbol CAPN1
Gene ID (NCBI) 823
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Calpain 1 antibody 10538-1-APDownload protocol
IHC protocol for Calpain 1 antibody 10538-1-APDownload protocol
IF protocol for Calpain 1 antibody 10538-1-APDownload protocol
IP protocol for Calpain 1 antibody 10538-1-APDownload protocol
FC protocol for Calpain 1 antibody 10538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Cell Death Dis

Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.

Authors - Xueyang Deng
humanWB

J Biol Chem

Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.

Authors - Hui-Yan Lei
human

Exp Ther Med

Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.

Authors - Pingheng Zhang
ratWB,IF

Ann Anat

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Authors - Shuchao Wang
humanWB

Anticancer Res

Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.

Authors - Urszula Ciesielska
humanWB,IHC,CoIP

Thorac Cancer

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Authors - Yichuan Chen
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (03-31-2021)

Antibody detected endogenous and overexpressed Calpain-1 in HEK cells

  • Applications: Western Blot,Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK cell overexpression
FH

Wei (Verified Customer) (03-12-2020)

Single, strong western blot band at predicted size.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart