|Positive WB detected in||A431 cells, A2780 cells, HeLa cells, Jurkat cells, K-562 cells, mouse testis tissue, PC-3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human urothelial carcinoma tissue, human heart tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10538-1-AP targets Calpain 1 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calpain 1 fusion protein Ag0810|
|Full Name||calpain 1, (mu/I) large subunit|
|Calculated molecular weight||704 aa, 82 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC008751|
|Gene ID (NCBI)||823|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IHC protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IF protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|IP protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|FC protocol for Calpain 1 antibody 10538-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (03-31-2021)
Antibody detected endogenous and overexpressed Calpain-1 in HEK cells
Wei (Verified Customer) (03-12-2020)
Single, strong western blot band at predicted size.