CAPN1 Monoclonal antibody

• Featured Product
• KD/KO Validated

CAPN1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

4E6H1

Cat no : 67732-1-Ig

Synonyms

Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

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Validation Data Gallery

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  WB analysis using 67732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67732-1-Ig

  WB result of CAPN1 antibody (67732-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPN1 transfected HeLa cells.

• 

  WB analysis using 67732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, LNCaP cells, T-47D cells, K562 cells
• Positive IHC detected in: human urothelial carcinoma tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

67732-1-Ig targets CAPN1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPN1 fusion protein Ag30522
• Full Name: calpain 1, (mu/I) large subunit
• Calculated Molecular Weight: 704 aa, 82 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC008751
• Gene Symbol: CAPN1
• Gene ID (NCBI): 823
• RRID: AB_2918501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.; Authors - Fengming Shen
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu
mouse	WB,IF	Neural Plast; Astragaloside IV Alleviates Brain Injury Induced by Hypoxia via the Calpain-1 Signaling Pathway; Authors - Yan Meng