CAPN1 Monoclonal antibody, PBS Only

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  WB analysis using 67732-1-Ig (same clone as 67732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67732-1-Ig (same clone as 67732-1-PBS)

  WB result of CAPN1 antibody (67732-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPN1 transfected HeLa cells. This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67732-1-Ig (same clone as 67732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67732-1-Ig (same clone as 67732-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67732-1-Ig (same clone as 67732-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67732-1-Ig (same clone as 67732-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67732-1-Ig (same clone as 67732-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CAPN1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67732-1-PBS

Clone No.4E6H1

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

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Product Information

67732-1-PBS targets CAPN1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30522

  Product name: Recombinant human CAPN1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 413-714 aa of BC008751

  Sequence: RESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFNALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

• Full Name: calpain 1, (mu/I) large subunit
• Calculated Molecular Weight: 704 aa, 82 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC008751
• Gene Symbol: CAPN1
• Gene ID (NCBI): 823
• RRID: AB_2918501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07384
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.