Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, LNCaP cells, HEK-293 cells, rat brain tissue, mouse brain tissue, HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue, human stomach cancer tissue, human colon cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 2 publications below |
Product Information
66977-1-Ig targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28427 Product name: Recombinant human CAPN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-330 aa of BC021303 Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD Predict reactive species |
| Full Name | calpain 2, (m/II) large subunit |
| Calculated Molecular Weight | 700 aa, 80 kDa |
| Observed Molecular Weight | 72-80 kDa |
| GenBank Accession Number | BC021303 |
| Gene Symbol | CAPN2 |
| Gene ID (NCBI) | 824 |
| RRID | AB_2882297 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P17655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928, and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from the human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPN2 antibody 66977-1-Ig | Download protocol |
| IHC protocol for CAPN2 antibody 66977-1-Ig | Download protocol |
| WB protocol for CAPN2 antibody 66977-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis. | ||
Cell Mol Life Sci Calpains as novel players in the molecular pathogenesis of spinocerebellar ataxia type 17. | ||
Int J Surg METTL16 inhibits pancreatic cancer proliferation and metastasis by promoting MROH8 RNA stability and inhibiting CAPN2 expression |
