• Featured Product
  • KD/KO Validated

CAPRIN2 Polyclonal antibody

CAPRIN2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 20766-1-AP

Synonyms

C1QDC1, caprin 2, caprin family member 2, CAPRIN2, EEG 1, EEG1, KIAA1873, Protein EEG 1, RNA granule protein 140, RNG140



Tested Applications

Positive WB detected inY79 cells, SH-SY5Y cells, HEK-293 cells
Positive IP detected inSH-SY5Y cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20766-1-AP is CAPRIN2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPRIN2 fusion protein Ag14745
Full Name caprin family member 2
Calculated molecular weight 1127 aa, 126 kDa
Observed molecular weight 126-150 kDa
GenBank accession numberBC007520
Gene symbol CAPRIN2
Gene ID (NCBI) 65981
RRIDAB_10695763
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IF protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IP protocol for CAPRIN2 antibody 20766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Neuroendocrinology

Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.

Authors - Soledad Bárez-López
mouseWB, IF

Dev Dyn

Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.

Authors - Soma Dash
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paul (Verified Customer) (04-29-2019)

Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T cells, Rat neuronal samples
CAPRIN2 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in HEK293T cells, Rat neuronal samples (Cat no:20766-1-AP)
FH

Criseyda (Verified Customer) (09-21-2018)

Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MRC-9 Lung Fiberblasts
CAPRIN2 Antibody Western Blot, validation (1:500 dilution) in MRC-9 Lung Fiberblasts (Cat no:20766-1-AP)