• Featured Product
  • KD/KO Validated

CAPRIN2 Polyclonal antibody

CAPRIN2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 20766-1-AP

Synonyms

C1QDC1, caprin 2, caprin family member 2, CAPRIN2, EEG 1, EEG1, KIAA1873, Protein EEG 1, RNA granule protein 140, RNG140



Tested Applications

Positive WB detected inY79 cells, SH-SY5Y cells, HEK-293 cells
Positive IP detected inSH-SY5Y cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20766-1-AP is CAPRIN2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPRIN2 fusion protein Ag14745
Full Name caprin family member 2
Calculated molecular weight 1127 aa, 126 kDa
Observed molecular weight 126-150 kDa
GenBank accession numberBC007520
Gene symbol CAPRIN2
Gene ID (NCBI) 65981
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IF protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IP protocol for CAPRIN2 antibody 20766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IF

Dev Dyn

Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.

Authors - Soma Dash
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paul (Verified Customer) (04-29-2019)

Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T cells, Rat neuronal samples
CAPRIN2 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in HEK293T cells, Rat neuronal samples (Cat no:20766-1-AP)
FH

Criseyda (Verified Customer) (09-21-2018)

Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MRC-9 Lung Fiberblasts
CAPRIN2 Antibody Western Blot, validation (1:500 dilution) in MRC-9 Lung Fiberblasts (Cat no:20766-1-AP)