|Positive WB detected in||Y79 cells, SH-SY5Y cells, HEK-293 cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20766-1-AP is CAPRIN2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CAPRIN2 fusion protein Ag14745|
|Full Name||caprin family member 2|
|Calculated molecular weight||1127 aa, 126 kDa|
|Observed molecular weight||126-150 kDa|
|GenBank accession number||BC007520|
|Gene ID (NCBI)||65981|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Paul (Verified Customer) (04-29-2019)
Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.
Criseyda (Verified Customer) (09-21-2018)
Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.