CAPRIN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAPRIN2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

2

Reviews (2)

Cat no : 20766-1-AP

Synonyms

C1QDC1, caprin 2, caprin family member 2, CAPRIN2, EEG 1, EEG1, KIAA1873, Protein EEG 1, RNA granule protein 140, RNG140

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Validation Data Gallery

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  WB analysis of Y79 using 20766-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20766-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of SH-SY5Y using 20766-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 20766-1-AP

  IP result of anti-CAPRIN2 (IP:20766-1-AP, 4ug; Detection:20766-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IF Staining of HepG2 using 20766-1-AP

  Immunofluorescent analysis of HepG2 cells using 20766-1-AP (CAPRIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 20766-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPRIN2 antibody 20766-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Y79 cells, SH-SY5Y cells, HEK-293 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20766-1-AP is CAPRIN2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAPRIN2 fusion protein Ag14745
• Full Name: caprin family member 2
• Calculated Molecular Weight: 1127 aa, 126 kDa
• Observed Molecular Weight: 126-150 kDa
• GenBank Accession Number: BC007520
• Gene Symbol: CAPRIN2
• Gene ID (NCBI): 65981
• RRID: AB_10695763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IHC	Neuroendocrinology; Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.; Authors - Soledad Bárez-López
mouse	WB, IF	Dev Dyn; Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.; Authors - Soma Dash ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paul (Verified Customer) (04-29-2019)

Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells, Rat neuronal samples

CAPRIN2 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in HEK293T cells, Rat neuronal samples (Cat no:20766-1-AP)

FH

Criseyda (Verified Customer) (09-21-2018)

Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MRC-9 Lung Fiberblasts

CAPRIN2 Antibody Western Blot, validation (1:500 dilution) in MRC-9 Lung Fiberblasts (Cat no:20766-1-AP)