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- KD/KO Validated
CAPRIN2 Polyclonal antibody
CAPRIN2 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF
Cat no : 20766-1-AP
|Positive WB detected in||Y79 cells, SH-SY5Y cells, HEK-293 cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 1 publications below|
|IHC||See 1 publications below|
|IF||See 1 publications below|
The immunogen of 20766-1-AP is CAPRIN2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CAPRIN2 fusion protein Ag14745|
|Full Name||caprin family member 2|
|Calculated molecular weight||1127 aa, 126 kDa|
|Observed molecular weight||126-150 kDa|
|GenBank accession number||BC007520|
|Gene ID (NCBI)||65981|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for CAPRIN2 antibody 20766-1-AP||Download protocol|
|IF protocol for CAPRIN2 antibody 20766-1-AP||Download protocol|
|IP protocol for CAPRIN2 antibody 20766-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.
Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Paul (Verified Customer) (04-29-2019)
Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.
Criseyda (Verified Customer) (09-21-2018)
Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.