CAPZA1 Monoclonal antibody

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• KD/KO Validated

CAPZA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

10H1F9

Cat no : 66066-1-Ig

Synonyms

capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66066-1-Ig

  WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells.

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  WB analysis using 66066-1-Ig

  Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66066-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66066-1-Ig (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 66066-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66066-1-Ig

  Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 66066-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CAPZA1 antibody (66066-1-Ig, Clone: 10H1F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of MCF-7 using 66066-1-Ig

  Immunofluorescent analysis of () fixed MCF-7 cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, ROS1728 cells, PC-12 cells, RAW 264.7 cells, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66066-1-Ig targets CAPZA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPZA1 fusion protein Ag18841
• Full Name: capping protein (actin filament) muscle Z-line, alpha 1
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC000144
• Gene Symbol: CAPZA1
• Gene ID (NCBI): 829
• RRID: AB_11042588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Publications

Species	Application	Title
human	IF	Cancers (Basel); Critical Role for Cold Shock Protein YB-1 in Cytokinesis.; Authors - Sunali Mehta