CoraLite® Plus 488-conjugated CAPZA1 Monoclonal antibody

CAPZA1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

10H1F9

Cat no : CL488-66066

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-66066

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL488-66066 (CAPZA1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66066 is CAPZA1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPZA1 fusion protein Ag18841
• Full Name: capping protein (actin filament) muscle Z-line, alpha 1
• Calculated Molecular Weight: 286 aa, 33 kDa
• GenBank Accession Number: BC000144
• Gene Symbol: CAPZA1
• Gene ID (NCBI): 829
• RRID: AB_2883248
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)