Tested Applications
| Positive WB detected in | A431 cells, mouse testis tissue, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human skin cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25043-1-AP targets CAPZB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19327 Product name: Recombinant human CAPZB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-243 aa of BC109241 Sequence: SDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLYFEGGVSSVYLWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTNKSGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIVNGL Predict reactive species |
| Full Name | capping protein (actin filament) muscle Z-line, beta |
| Calculated Molecular Weight | 277 aa, 31 kDa |
| Observed Molecular Weight | 30-31 kDa |
| GenBank Accession Number | BC109241 |
| Gene Symbol | CAPZB |
| Gene ID (NCBI) | 832 |
| RRID | AB_2879867 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47756 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CAPZB belongs to the F-actin-capping protein beta subunit family and is the β subunit of the heterodimeric F-actin cap-binding protein. F-actin-capping proteins bind in a Ca2+-independent manner to the fast-growing ends of actin filaments, thereby blocking the exchange of subunits at these ends.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPZB antibody 25043-1-AP | Download protocol |
| IHC protocol for CAPZB antibody 25043-1-AP | Download protocol |
| WB protocol for CAPZB antibody 25043-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CRAD antibody (Cat# 25043-1-AP) has 10 total citations. Citations appear in Molecular Medicine, Cell Reports (×3), Communications Biology, BMC Biology, Scientific Reports, Development, eLife, and bioRxiv. Research fields span hypertrophic cardiomyopathy, sperm head morphology, colorectal tumorigenic stemness, actin cytoskeleton architecture in embryonic stem cells, tumor progression, endosomal trafficking and viral entry, aortic dissection immune infiltration, male subfertility, Alzheimer's disease interactome mapping, and F-actin regulation of endocytic vesicles.
| Species | Application | Title |
|---|---|---|
Mol Med Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy | ||
Cell Rep Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction. | ||
Cell Rep Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.
| ||
Cell Rep The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice. | ||
Commun Biol Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton. | ||
BMC Biol The temporal association of CapZ with early endosomes regulates endosomal trafficking and viral entry into host cells |
