CBS Monoclonal antibody

CBS Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E4F10

Cat no : 67861-1-Ig

Synonyms

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase



Tested Applications

Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67861-1-Ig targets CBS in WB, IHC,ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CBS fusion protein Ag7008
Full Name cystathionine-beta-synthase
Calculated molecular weight 61 kDa
Observed molecular weight 61-63 kDa
GenBank accession numberBC000440
Gene symbol CBS
Gene ID (NCBI) 875
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Protocols

Product Specific Protocols
WB protocol for CBS antibody 67861-1-IgDownload protocol
IHC protocol for CBS antibody 67861-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols