Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue, HeLa cells, MCF-7 cells, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15579-1-AP targets CBX2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7979 Product name: Recombinant human CBX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC004252 Sequence: MEELSSVGEQVFAAECILSKRLRKGKLEYLVKWRGWSSKHNSWEPEENILDPRLLLAFQKKEHEKEVQNRKRGKRPRGRPRKLTAMSSCSRRSKLKVGGCAGYADPTSQHPLGVGGRQREGLGPSGRGWHFCQQSVPLLGKQEPPFFLSLSFCCQGPQPAESSSPPLPGASCFSLSCTPLCWVAGSNCCRQALFPPRGSLGDGKEQEACVQ Predict reactive species |
| Full Name | chromobox homolog 2 (Pc class homolog, Drosophila) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC004252 |
| Gene Symbol | CBX2 |
| Gene ID (NCBI) | 84733 |
| RRID | AB_2737362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14781 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The chromobox homologue protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBX2 antibody 15579-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CBX2 antibody (Cat# 15579-1-AP) has 15 total citations spanning journals including Cell, Oncogene, Cell Death & Disease, Cell Reports, Molecular Cancer Therapeutics, Cell Signaling, BMC Cancer, Heliyon, Molecular Carcinogenesis, Journal of Cancer, Scientific Reports, Oncology Letters, American Journal of Translational Research, and Cancer Letters. Research fields cover epigenetics, chromatin regulation, and multiple cancer types (ovarian, colorectal, gastric, breast, hepatocellular, bladder, neuroblastoma), with emphasis on drug resistance and signaling pathways.
| Species | Application | Title |
|---|---|---|
Cell Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.
| ||
Cell Death Dis CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
| ||
Oncogene USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2 | ||
Oncogene CBX2 as a therapeutic target in colorectal cancer: insights into the altered chromatin accessibility via RUNX1-CBX2-MAP4K1 axis
| ||
Mol Cancer Ther Chromobox2 inhibition: a novel activity of alisertib, an aurora A kinase inhibitor. |
