CBX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CBX2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 15579-1-AP

Synonyms

CBX2, CDCA6, Chromobox protein homolog 2, M33

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Validation Data Gallery

• 

  WB analysis using 15579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15579-1-AP (CBX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15579-1-AP (CBX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15579-1-AP (CBX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, HeLa cells, MCF-7 cells, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

15579-1-AP targets CBX2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBX2 fusion protein Ag7979
• Full Name: chromobox homolog 2 (Pc class homolog, Drosophila)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC004252
• Gene Symbol: CBX2
• Gene ID (NCBI): 84733
• RRID: AB_2737362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The chromobox homologue protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.

Publications

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang ; KD Validated
human	WB,IHC	Am J Transl Res; Overexpression of CBX2 in breast cancer promotes tumor progression through the PI3K/AKT signaling pathway.; Authors - Shipeng Zheng ; KD Validated
human	WB,IHC,IF	Mol Carcinog; Loss of CBX2 causes genomic instability and Wnt activation in high grade serous ovarian carcinoma cells; Authors - Yujia Ma ; KD Validated
mouse	WB	Cell Rep; SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions; Authors - Haining Zhou
human	WB	Oncol Lett; Comprehensive analysis reveals the value of the expression of chromobox family members for bladder urothelial carcinoma prognosis; Authors - Xuan Meng
human	WB	Heliyon; Derepressing of STAT3 and USP7 contributes to resistance of DLBCL to EZH2 inhibition; Authors - Chenyun He