Validation Data Gallery
|Positive WB detected in
|mouse spleen tissue, A375 cells
|Positive IP detected in
|mouse spleen tissue
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11650-2-AP targets CBX3 in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|CBX3 fusion protein Ag2251
|chromobox homolog 3 (HP1 gamma homolog, Drosophila)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins
|Product Specific Protocols
|WB protocol for CBX3 antibody 11650-2-AP
|IHC protocol for CBX3 antibody 11650-2-AP
|IF protocol for CBX3 antibody 11650-2-AP
|IP protocol for CBX3 antibody 11650-2-AP
|Click here to view our Standard Protocols
Retrospective analysis of enhancer activity and transcriptome history
Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.
Adv Sci (Weinh)
Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer
J Exp Med
ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.
The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.
J Cell Biol
Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (05-11-2022)
Strong bands for predicted size.