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  • KD/KO Validated

CBX3 Polyclonal antibody

CBX3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 11650-2-AP

Synonyms

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein



Tested Applications

Positive WB detected inmouse spleen tissue, A375 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11650-2-AP targets CBX3 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX3 fusion protein Ag2251
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000954
Gene symbol CBX3
Gene ID (NCBI) 11335
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Protocols

Product Specific Protocols
WB protocol for CBX3 antibody 11650-2-APDownload protocol
IHC protocol for CBX3 antibody 11650-2-APDownload protocol
IF protocol for CBX3 antibody 11650-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Gene

CBX3 promotes tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma.

Authors - HuaYong Zhang
  • KD Validated
humanIHC

Lung Cancer

RNA sequencing identifies novel markers of non-small cell lung cancer.

Authors - Seon-Sook Han
humanWB

J Virol

PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.

Authors - Zhangping He
humanWB

Mol Neurobiol

The LncRNA RP11-279C4.1 Enhances the Malignant Behaviour of Glioma Cells and Glioma Stem-Like Cells by Regulating the miR-1273g-3p/CBX3 Axis.

Authors - Faming Wang
humanWB,IF

Cancer Gene Ther

PCA3 controls chromatin organization and p53 signal activation by regulating LAP2α-lamin A complexes.

Authors - Saya Ito
ratIF

Stem Cell Res Ther

A pH probe inhibits senescence in mesenchymal stem cells.

Authors - Lihong Wang