CBY1 Polyclonal antibody

CBY1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 12239-1-AP

Synonyms

arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1

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Validation Data Gallery

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  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12239-1-AP

  IP result of anti-CBY1 (IP:12239-1-AP, 4ug; Detection:12239-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12239-1-AP targets CBY1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBY1 fusion protein Ag2882
• Full Name: chibby homolog 1 (Drosophila)
• Calculated Molecular Weight: 126 aa, 14 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC016139
• Gene Symbol: CBY1
• Gene ID (NCBI): 25776
• RRID: AB_10951858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
mouse,human	WB,IF	Dev Dyn; DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.; Authors - Lilong Guo
human	IHC	Medicine (Baltimore); Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma; Authors - Gang Ren
human	IF	Adv Sci (Weinh); The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers; Authors - Huicheng Chen
human	WB	Heliyon; MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling; Authors - Qingqing Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Feng-Qian (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T