CBY1 Polyclonal antibody

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• CBY1 Polyclonal antibody (12239-1-AP)

CBY1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12239-1-AP Copied!

Publications (7)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1

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  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 12239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 12239-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 12239-1-AP

  IP result of anti-CBY1 (IP:12239-1-AP, 4ug; Detection:12239-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 12239-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12239-1-AP targets CBY1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2882

  Product name: Recombinant human CBY1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-126 aa of BC016139

  Sequence: GSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK

• Full Name: chibby homolog 1 (Drosophila)
• Calculated Molecular Weight: 126 aa, 14 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC016139
• Gene Symbol: CBY1
• Gene ID (NCBI): 25776
• RRID: AB_10951858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y3M2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CBY1 Polyclonal antibody (Cat# 12239-1-AP) has 7 citations published in Advanced Science, Journal of Cell Biology, PeerJ, Medicine (Baltimore), Developmental Dynamics, medRxiv, and Heliyon. Research fields span ciliary biology, airway cell differentiation, endoplasmic reticulum stress in cancer, laryngeal squamous cell carcinoma, cardiac valve development, ovarian cancer, and EMT/cisplatin resistance. Applications cited include WB, IHC, and IF in human and mouse samples.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Adv Sci (Weinh); The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers; Authors - Huicheng Chen
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
human	IHC	PeerJ; Multi-omics evidence reveals a causal role of endoplasmic reticulum stress in cancer development.; Authors - Rui Guo
human	IHC	Medicine (Baltimore); Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma; Authors - Gang Ren
mouse,human	WB,IF	Dev Dyn; DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.; Authors - Lilong Guo
human	WB	Heliyon; MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling; Authors - Qingqing Chen

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single available review for this product is a 5-star rating from a Western Blot application using HEK293T cells at a 1/1000 dilution. The reviewer did not leave written comments or upload an image, but the top rating indicates strong satisfaction with the antibody's performance under those conditions. Overall, the limited review base suggests the product is well-received, though broader user feedback is not yet available.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Feng-Qian (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T