CCBL1/KAT1 Polyclonal antibody

• 

  WB analysis of mouse kidney using 12156-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12156-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12156-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12156-1-AP (CCBL1/KAT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 12156-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12156-1-AP (CCBL1/KAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCBL1/KAT1 Polyclonal Antibody for WB, IF, ELISA

Cat No. 12156-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCBL1, CCBL1/KAT1, Glutamine transaminase K, GTK, KAT1, KATI, KYAT1, Kynurenine aminotransferase I

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells, L02 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

12156-1-AP targets CCBL1/KAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2828

  Product name: Recombinant human CCBL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-112 aa of BC013069

  Sequence: MRHHTRPIFLFLVETGFSHVGQAGLELLTSGDPPVSASQSAGIIGVSHRTWPCSSDFDAALGAGLELTLLPRLVVGTSLSPPEPRPAGPRGRVETCPSSSPGLGGHPCLHLL

• Full Name: cysteine conjugate-beta lyase, cytoplasmic
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC013069
• Gene Symbol: CCBL1
• Gene ID (NCBI): 883
• RRID: AB_10666849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16773
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression.; Authors - Leandro Z Agudelo
human	WB,IHC	J Intern Med; Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.; Authors - R Baumgartner
mouse	WB	Brain Behav Immun; Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise.; Authors - Alessandro Ieraci
human	WB	Toxicology; Cannabidiol disrupts tryptophan metabolism in the human term placenta; Authors - Ramon Portillo
		Am J Physiol Cell Physiol; Endurance exercise increases skeletal muscle kynurenine aminotransferases and plasma kynurenic acid in humans.; Authors - Maja Schlittler
mouse	WB	Sci Rep; Caffeine protects against stress-induced murine depression through activation of PPARγC1α-mediated restoration of the kynurenine pathway in the skeletal muscle.; Authors - Chongye Fang