CCBL2 Polyclonal antibody

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  WB analysis using 55476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55476-1-AP (CCBL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 55476-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCBL2 antibody (55476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCBL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 55476-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RBMXL1, KATIII, KAT3, GTL, Glutamine Transaminase L

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55476-1-AP targets CCBL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cysteine conjugate-beta lyase 2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48 kDa，51- 55 kda
• GenBank Accession Number: NM_019610
• Gene Symbol: CCBL2
• Gene ID (NCBI): 56267
• RRID: AB_3670254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6YP21
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cysteine conjugate beta-lyase 2 (CCBL2), also known as kynurenine aminotransferase 3 (KAT3) or glutamine transaminase L (GTL), can effectively catalyze the transamination of glutamine, methionine, and kynurenine (KYN) to kynurenic acid (KYNA) as well as the pathway of drug metabolism by cytochrome P450. CCBL2 has been found in mouse, rat, and human whose mRNA is widely expressed in several organs such as the liver, kidney, heart, and neuroendocrine tissues (PMID: 35771747).