SGF29 Polyclonal antibody

• Featured Product
• KD/KO Validated

SGF29 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24061-1-AP

Synonyms

CCDC101, SGF29

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Validation Data Gallery

• 

  WB analysis of HeLa using 24061-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 24061-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human liver cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human cervical cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human cervical cancer using 24061-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-453s cells
• Positive IHC detected in: human liver cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

24061-1-AP targets SGF29 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGF29 fusion protein Ag21261
• Full Name: coiled-coil domain containing 101
• Calculated Molecular Weight: 293 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC011981
• Gene Symbol: CCDC101
• Gene ID (NCBI): 112869
• RRID: AB_2879420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

Publications

Species	Application	Title
human	WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long ; KO Validated