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CCDC136 Polyclonal antibody, PBS Only (21351-1-PBS)

Filter: AllWBIPIHC

WB analysis using 21351-1-AP (same clone as 21351-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21351-1-AP (CCDC136 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)
IP result of anti-CCDC136 (IP:21351-1-AP, 4ug; Detection:21351-1-AP 1:800) with mouse testis tissue lysate 1800 ug. This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 21351-1-AP (same clone as 21351-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 21351-1-AP (same clone as 21351-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

CCDC136 Polyclonal antibody, PBS Only

Name: CCDC136 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21351-1-PBS

CCDC136 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 21351-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21351-1-PBS targets CCDC136 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15737 Product name: Recombinant human CCDC136 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 461-810 aa of BC109220 Sequence: AELQHLRDTVASFKESNEKDTETHAQLQEMKQLYQASKDELERQKHMYDQLEQDLLLCQLELKELKASHPIPEDKGKCANKCDTLLSRLTELQEKYKASQKEMGQLQMEQCELLEDQRRMQEEQGQLQEELHRLTLPLPKSGLLLKSQELLTKLEDLCELQLLYQGMQEEQKKLIQNQDCVLKEQLEIHEELRRFKESHFQEVLENPDDSKLAKSSKCNRNKQSKLLMEQMQALQVMYDAGQAKQELLQQEQGRLLEERKRLQADLQLCLEEMQLLQVQSPSIKMSLESYGKSYGSMVPSNENCRKTYDTTVDDNESYYKSYTSTQTSSKSFLKSYDSSTSASEAYGKSY Predict reactive species
Full Name	coiled-coil domain containing 136
Calculated Molecular Weight	1154 aa, 134 kDa
Observed Molecular Weight	110 kDa, 134 kDa
GenBank Accession Number	BC109220
Gene Symbol	CCDC136
Gene ID (NCBI)	64753
RRID	AB_3742029
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96JN2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CCDC136 (also known as NAG6) is a coiled-coil domain containing protein described as a potential tumor suppressor in gastric cancer. CCDC136 protein was most abundantly located in the acrosome of round spermatids and elongating spermatids within seminiferous tubules of the adult mouse testis (PMID: 27076447).