CCDC158 Polyclonal antibody

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• CCDC158 Polyclonal antibody (20396-1-AP)

CCDC158 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20396-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Coiled-coil domain-containing protein 158

• 

  WB analysis using 20396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 20396-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 20396-1-AP

  IP result of anti-CCDC158 (IP:20396-1-AP, 4ug; Detection:20396-1-AP 1:500) with mouse testis tissue lysate 6000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20396-1-AP targets CCDC158 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14049

  Product name: Recombinant human CCDC158 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-201 aa of BC035224

  Sequence: MESKAWESNNEDLLSSSGVTSNGGSSSSFFVSSIRGTIIENTSSAGTLTQVPFFPKYEVELDSPRKIIPSPGKEHFERVLEEYSHQVKDLQRRLNESNELHEKQKFYLRQSVIDLQTKLQEMQMERDAMADIRRRESQSQEDLRNQLQNTVHELEAAKCLKEDMLKDSNTQIEQLRKMMLSHEGVLQEIRSILVDFEEASG

• Full Name: coiled-coil domain containing 158
• Calculated Molecular Weight: 1113 aa, 127 kDa
• Observed Molecular Weight: 127-140 kDa
• GenBank Accession Number: BC035224
• Gene Symbol: CCDC158
• Gene ID (NCBI): 339965
• RRID: AB_10694278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5M9N0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CCDC158 Polyclonal antibody (Cat# 20396-1-AP) has 1 citation to date. It appears in the Journal of Cellular Physiology (2024), in a study investigating CCDC158 as a novel regulator in renal proximal tubular endocytosis, identified through exome sequencing and interactome analysis. The research field encompasses cell physiology, kidney biology, and protein interactome studies. The antibody was applied via IHC and IF in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC,IF	J Cell Physiol; CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis; Authors - Tjessa Bondue