CCDC25 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCDC25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 21209-1-AP

Synonyms

Coiled-coil domain-containing protein 25

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Validation Data Gallery

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  WB analysis using 21209-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21209-1-AP (CCDC25 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 21209-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21209-1-AP (CCDC25 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

21209-1-AP targets CCDC25 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC25 fusion protein Ag15593
• Full Name: coiled-coil domain containing 25
• Calculated Molecular Weight: 208 aa, 24 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC032588
• Gene Symbol: CCDC25
• Gene ID (NCBI): 55246
• RRID: AB_10732690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF,WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang ; KO Validated
human,mouse	WB,IF	Nat Cell Biol; Eosinophil extracellular traps drive asthma progression through neuro-immune signals.; Authors - Yiwen Lu ; KO Validated
human	WB,IHC	ACS Nano; Choline Phosphate-Grafted Nanozymes as Universal Extracellular Vesicle Probes for Bladder Cancer Detection; Authors - Qiang Li
human	IP	FASEB J; Coiled-coil domain containing 25 (CCDC25) regulates cell proliferation, migration, and invasion in clear cell renal cell carcinoma by targeting the ILK-NF-κB signaling pathway; Authors - Zhenzhen Qian
mouse	WB,IF	Dev Neurobiol; Ccdc25 regulates neurogenesis during the brain development; Authors - Chong Wang
mouse	WB	J Control Release; Cell membrane derived liposomes loaded with DNase I target neutrophil extracellular traps which inhibits colorectal cancer liver metastases; Authors - Zhaozhong Wang