CCDC28A Recombinant monoclonal antibody

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• CCDC28A Recombinant monoclonal antibody (86883-1-RR)

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  WB analysis using 86883-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86883-1-RR (CCDC28A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 86883-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86883-1-RR (CCDC28A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 86883-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (86883-1-RR, Clone: 251922E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86883-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86883-1-RR against Human CCDC28A were performed. The affinity constant is below 1 pM.

CCDC28A Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86883-1-RR

Clone No.251922E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A

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Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells, A375 cells, mouse thymus tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86883-1-RR targets CCDC28A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6529

  Product name: Recombinant human CCDC28A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-162 aa of BC000758

  Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS

• Full Name: coiled-coil domain containing 28A
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000758
• Gene Symbol: CCDC28A
• Gene ID (NCBI): 25901
• RRID: AB_3745184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IWP9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).