CEP112 Polyclonal antibody

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  WB analysis of HEK-293 using 24928-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24928-1-AP (CEP112 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24928-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24928-1-AP (CEP112 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 24928-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP112 antibody (24928-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

CEP112 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24928-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IF/ICC, ELISA

Synonyms

CCDC46, Coiled-coil domain-containing protein 46, Centrosomal protein of 112 kDa

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Product Information

24928-1-AP targets CEP112 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP112 fusion protein Ag21586
• Full Name: coiled-coil domain containing 46
• Calculated Molecular Weight: 955 aa, 113 kDa
• Observed Molecular Weight: 110-113 kDa
• GenBank Accession Number: BC131805
• Gene Symbol: CEP112
• Gene ID (NCBI): 201134
• RRID: AB_2879802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N8E3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen ; KO Validated
mouse	WB,IF	PLoS Biol; Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.; Authors - Suchismita Panda
human	WB,IF	Clin Genet; Loss-of-function mutations in centrosomal protein 112 is associated with human acephalic spermatozoa phenotype.; Authors - Yanwei Sha

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (11-08-2019)

Human RPE1 cell line with Methanol fixationImmunostaining result shows that CEP112 localizes to centrosome.(Red;Cep112, Green; Cep164 for Centrosome marker, Blue;DAPI for Nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human RPE1

CEP112 Antibody Immunofluorescence, validation (1:100 dilution) in Human RPE1 (Cat no:24928-1-AP)