CCDC51 Polyclonal antibody

• 

  WB analysis using 20465-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20465-1-AP (CCDC51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 20465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20465-1-AP (CCDC51 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20465-1-AP (CCDC51 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20465-1-AP (CCDC51 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CCDC51 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20465-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MITOK, Mitochondrial potassium channel, Coiled-coil domain-containing protein 51

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

20465-1-AP targets CCDC51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14301

  Product name: Recombinant human CCDC51 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 110-399 aa of BC004341

  Sequence: MVARGLVREAREDLEVHQAKLKEVRDRLDRVSREDSQYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPVSLQEAIREQASSYSRQQRDLHNLMVDLRGLVHAAGPGQDSGSQAGSPPTRDRDVDVLSAALKEQLSHSRQVHSCLEGLREQLDGLEKTCSQMAGVVQLVKSAAHPGLVEPADGAMPSFLLEQGSMILALSDTEQRLEAQVNRNTIYSTLVTCVTFVA

• Full Name: coiled-coil domain containing 51
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC004341
• Gene Symbol: CCDC51
• Gene ID (NCBI): 79714
• RRID: AB_10700001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96ER9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF,WB	bioRxiv; Human CCDC51 and yeast Mdm33 are functionally conserved mitochondrial inner membrane proteins that demarcate a subset of organelle fission events; Authors - Alia R Edington ; KD Validated
human	WB,IF	J Cell Biol; Functionally conserved inner mitochondrial membrane proteins CCDC51 and Mdm33 demarcate a subset of fission events; Authors - Alia R Edington
mouse	IHC	Int J Mol Sci; SARS-CoV-2-ORF-3a Mediates Apoptosis Through Mitochondrial Dysfunction Modulated by the K+ Ion Channel; Authors - Muhammad Suhaib Qudus
		Acta Biochim Biophys Sin (Shanghai); Dexmedetomidine ameliorates high glucose-induced epithelial-mesenchymal transformation in HK-2 cells through the Cdk5/Drp1/ROS pathway; Authors - Fei Wang