CCDC66 Polyclonal antibody

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• CCDC66 Polyclonal antibody (26333-1-AP)

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  WB analysis using 26333-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 26333-1-AP (CCDC66 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

CCDC66 Polyclonal Antibody for WB, ELISA

Cat No. 26333-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Coiled-coil domain-containing protein 66

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Tested Applications

• Positive WB detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26333-1-AP targets CCDC66 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23421

  Product name: Recombinant human CCDC66 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-364 aa of BC132827

  Sequence: MTFSSTKDLCKQCIDKDCLHIQKEISPATPNMQKTRNTVNTSLVGKQKPHKKHITAENMKSSLVCLTQDQLQQILMTVNQGNRSLSLTENGKEAKSQYSLYLNSISNQPKDENIMGLFKKTEMVSSVPAENKSVLNEHQETSKQCEQKIAIENEWKPADIFSTLGERECDRSSLEAKKAQWRKELDEQVALKKKEKEVSEKWNDPWKKSESDKIIWEKHQILDQSRETVLLEHPFSAVKQELQRKWIEELNKQIEDDRQRKIEEKIIYSKGEEHDRWAMHFDSLKSYPGSQSQLFSRSTHKQPEYFCVSPDTQELADVSSVCTPTTGSQVEPSEEEHIAKPIKDVVMANSKKTNFLRSMTALLD

• Full Name: coiled-coil domain containing 66
• Observed Molecular Weight: 105-125 kDa
• GenBank Accession Number: BC132827
• Gene Symbol: CCDC66
• Gene ID (NCBI): 285331
• RRID: AB_3742183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A2RUB6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC66 is a MAP that localizes to the centriolar satellites, the centrosome and the primary cilium and is linked in ciliopathies affecting retina and brain. It interacts with the ciliopathy proteins CEP104 and CSPP1 and controls axonemal elongation (PMID: 36606424). CCDC66 is required for the basal body recruitment of transition zone proteins and intraflagellar transport B (IFT-B) machinery.