CCDC88A Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CCDC88A Polyclonal antibody (33569-1-AP)

• 

  WB analysis using 33569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33569-1-AP (CCDC88A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CCDC88A Polyclonal Antibody for WB, ELISA

Cat No. 33569-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Akt phosphorylation enhancer, APE, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, Girders of actin filament

---

Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33569-1-AP targets CCDC88A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37983

  Product name: Recombinant human CCDC88A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1500-1843 aa of BC132736

  Sequence: GAMAFSTTAINFSTVNSSAGFRSKQLVNNKDTTSFEDISPQGVSDDSSTGSRVHASRPASLDSGRTSTSNSNNNASLHEVKAGAVNNQSRPQSHSSGEFSLLHDHEAWSSSGSSPIQYLKRQTRSSPVLQHKISETLESRHHKIKTGSPGSEVVTLQQFLEESNKLTSVQIKSSSQENLLDEVMKSLSVSSDFLGKDKPVSCGLARSVSGKTPGDFYDRRTTKPEFLRPGPRKTEDTYFISSAGKPTPGTQGKIKLVKESSLSRQSKDSNPYATLPRASSVISTAEGTTRRTSIHDFLTKDSRLPISVDSPPAAADSNTTAASNVDKVQESRNSKSRSREQQSS

• Full Name: coiled-coil domain containing 88A
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC132736
• Gene Symbol: CCDC88A
• Gene ID (NCBI): 55704
• RRID: AB_3743013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q3V6T2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC88A, also named as APE, GRDN and KIAA1212, belongs to the CCDC88 family. CCDC88A enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby it induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation CCDC88A is essential for the integrity of the actin cytoskeleton and for cell migration. It is required for formation of actin stress fibers and lamellipodia. It may be involved in membrane sorting in the early endosome.