Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HEK293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human ovary cancer tissue, rat testis tissue, mouse testis tissue, rat ovary tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24689-1-AP targets CCDC99 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20430 Product name: Recombinant human CCDC99 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 259-605 aa of BC012568 Sequence: AEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLEKSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKSHPILYVSSKSTPETQCPQQ Predict reactive species |
| Full Name | coiled-coil domain containing 99 |
| Calculated Molecular Weight | 605 aa, 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC012568 |
| Gene Symbol | CCDC99 |
| Gene ID (NCBI) | 54908 |
| RRID | AB_2879673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCDC99 antibody 24689-1-AP | Download protocol |
| IP protocol for CCDC99 antibody 24689-1-AP | Download protocol |
| WB protocol for CCDC99 antibody 24689-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCDC99 Polyclonal antibody (Cat# 24689-1-AP) has 3 total citations. These appear in Cells, Molecular Biology of the Cell, and PLoS One. The cited research spans protein–protein interaction mapping (Ku70 interactors), kinetochore–microtubule attachment and chromosome segregation, and oncology, specifically the role of CCDC99 in esophageal squamous cell carcinoma growth, metastasis, and tumor immune microenvironment. Applications cited include Western blot and immunofluorescence in human samples.
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Dynamic Phosphorylation of MIS12 Ensures Accurate Kinetochore-Microtubule Attachment by Expanding the Fibrous Corona | ||
PLoS One Inhibiting the expression of spindle appendix cooled coil protein 1 can suppress tumor cell growth and metastasis and is associated with cancer immune cells in esophageal squamous cell carcinoma
|
Reviews
What customers say
The single review rates this antibody 4 out of 5 stars. The user found it performs well for Western Blotting at a 1:1000 dilution, producing strong signal with minimal background. However, for Immunofluorescence at 1:100 dilution on HeLa cells, the antibody failed to produce a clear specific signal, showing only background. Overall, the antibody is recommended for WB applications but not for IF.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Martijn (Verified Customer) (03-02-2023) | Antibody seems to work really well for western blotting, the signal is strong, and it doesn't show a lot of background. For immunofluorescence it does not give a clear signal, and it looks like it only shows background
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