• Featured Product
  • KD/KO Validated

CCDC99 Polyclonal antibody

CCDC99 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24689-1-AP

Synonyms

CCDC99, hSpindly, MU-RMS-40.4A, Protein Spindly, SPDL1, spdly



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24689-1-AP targets CCDC99 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC99 fusion protein Ag20430
Full Name coiled-coil domain containing 99
Calculated Molecular Weight 605 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC012568
Gene Symbol CCDC99
Gene ID (NCBI) 54908
RRIDAB_2879673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCDC99 antibody 24689-1-APDownload protocol
IHC protocol for CCDC99 antibody 24689-1-APDownload protocol
IP protocol for CCDC99 antibody 24689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Cells

Identification of Ku70 Domain-Specific Interactors Using BioID2.

Authors - Sanna Abbasi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Martijn (Verified Customer) (03-02-2023)

Antibody seems to work really well for western blotting, the signal is strong, and it doesn't show a lot of background. For immunofluorescence it does not give a clear signal, and it looks like it only shows background

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WB: 1:1000, IF: 1:100
  • Cell Tissue Type: HeLa
CCDC99 Antibody Western Blot,Immunofluorescence validation (WB: 1:1000, IF: 1:100 dilution) in HeLa (Cat no:24689-1-AP)