CCKBR-specific Polyclonal antibody, PBS Only

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• CCKBR-specific Polyclonal antibody, PBS Only (16549-1-PBS)

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  WB analysis of BxPC-3 using 16549-1-AP (same clone as 16549-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 16549-1-AP (same clone as 16549-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 16549-1-AP (same clone as 16549-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16549-1-AP (same clone as 16549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 16549-1-AP (same clone as 16549-1-PBS)

  Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

CCKBR-specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16549-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CCKBR, CCK B, CCK B receptor, CCK BR, CCK2 R

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Product Information

16549-1-PBS targets CCKBR-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cholecystokinin B receptor
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 64 kDa, 50 kDa
• GenBank Accession Number: NM_176875
• Gene Symbol: CCKBR
• Gene ID (NCBI): 887
• RRID: AB_2070508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32239
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.