Anti-Mouse CCL11/Eotaxin Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98464-1-RR

  1x10^6 HEK-293T cells (left) CCL11/Eotaxin-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL11/Eotaxin Rabbit RecAb (98464-1-RR, Clone: 243012F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98464-1-RR

  1x10^6 HEK-293T cells (left) or CCL11/Eotaxin-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL11/Eotaxin Rabbit RecAb (98464-1-RR, Clone: 243012F8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CCL11/Eotaxin Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98464-1-RR

Clone No.243012F8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

C-C motif chemokine 11, Ccl11, Eosinophil chemotactic protein, Eotaxin, Scya11

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98464-1-RR targets CCL11/Eotaxin in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCL11/Eotaxin fusion protein Eg2400
• Full Name: chemokine (C-C motif) ligand 11
• Calculated Molecular Weight: 11kd
• GenBank Accession Number: NM_011330
• Gene Symbol: Ccl11
• Gene ID (NCBI): 20292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48298
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CCL11 also named Eotaxin-1, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.