Anti-Mouse CCL11/Eotaxin Rabbit Recombinant Antibody, PBS Only

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  FC experiment of Transfected HEK-293T using 98464-1-RR (same clone as 98464-1-PBS)

  1x10^6 HEK-293T cells (left) CCL11/Eotaxin-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL11/Eotaxin Rabbit RecAb (98464-1-RR, Clone: 243012F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98464-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98464-1-RR (same clone as 98464-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL11/Eotaxin-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL11/Eotaxin Rabbit RecAb (98464-1-RR, Clone: 243012F8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98464-1-PBS in a different storage buffer formulation.

CCL11/Eotaxin Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98464-1-PBS

Clone No.243012F8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

C-C motif chemokine 11, Ccl11, Eosinophil chemotactic protein, Eotaxin, Scya11

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Product Information

98464-1-PBS targets CCL11/Eotaxin in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCL11/Eotaxin fusion protein Eg2400
• Full Name: chemokine (C-C motif) ligand 11
• Calculated Molecular Weight: 11kd
• GenBank Accession Number: NM_011330
• Gene Symbol: Ccl11
• Gene ID (NCBI): 20292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48298
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CCL11 also named Eotaxin-1, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.