CCL12/MCP-5 Recombinant monoclonal antibody

• 

  WB analysis using 86565-1-RR

  Non-transfected and CCL12-transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 86565-1-RR (Ccl12 antibody) at a dilution of 1:5000, incubated at room temperature for 1.5 hours.

CCL12/MCP-5 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86565-1-RR

Clone No.250386A9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

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Tested Applications

• Positive WB detected in: Transfected with Mouse Ccl12 Transfected HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86565-1-RR targets CCL12/MCP-5 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3315

  Product name: Recombinant Mouse CCL12/MCP-5 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 23-104 aa of NM_011331.2

  Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

• Full Name: chemokine (C-C motif) ligand 12
• Calculated Molecular Weight: 12KD
• GenBank Accession Number: NM_011331.2
• Gene Symbol: Ccl12
• Gene ID (NCBI): 20293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62401
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.