Anti-Mouse CCL12/MCP-5 Rabbit Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• Anti-Mouse CCL12/MCP-5 Rabbit Recombinant Antibody (98542-1-RR)

CCL12/MCP-5 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98542-1-RR Copied!

Clone No. 250386G10

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

• 

  FC experiment of Transfected HEK-293T using 98542-1-RR

  1x10^6 HEK-293T cells and CCL12/MCP-5-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse Ccl12 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected HEK-293T using 98542-1-RR

  1x10^6 HEK-293T cells (left) or CCL12/MCP-5-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL12/MCP-5 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98542-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98542-1-RR against Mouse CCL12/MCP-5 were performed. The affinity constant is 94.9 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CCL12/MCP-5 in ?

Chat with Able

---

Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98542-1-RR targets CCL12/MCP-5 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3315

  Product name: Recombinant Mouse CCL12/MCP-5 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 23-104 aa of NM_011331.2

  Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

• Full Name: chemokine (C-C motif) ligand 12
• Calculated Molecular Weight: 12KD
• GenBank Accession Number: NM_011331.2
• Gene Symbol: Ccl12
• Gene ID (NCBI): 20293
• RRID: AB_3746167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62401
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.