CCL20/MIP-3 alpha Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 84413-3-RR (same clone as 84413-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84413-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 84413-3-RR (same clone as 84413-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84413-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 84413-3-RR (same clone as 84413-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84413-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01301-1

  Cytometric bead array standard curve of MP01301-1, CCL20/MIP-3 alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84413-1-PBS. Detection antibody: 84413-3-PBS. Standard: Eg2398. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01301-3

  Cytometric bead array standard curve of MP01301-3, CCL20/MIP-3 alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84413-3-PBS. Detection antibody: 84413-2-PBS. Standard: Eg2398. Range: 0.781-100 ng/mL.

CCL20/MIP-3 alpha Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 84413-3-PBS

Clone No.241694B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20

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Product Information

84413-3-PBS targets CCL20/MIP-3 alpha as part of a matched antibody pair:

MP01301-1: 84413-1-PBS capture and 84413-3-PBS detection (validated in Cytometric bead array)

MP01301-3: 84413-3-PBS capture and 84413-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-C motif) ligand 20
• Calculated Molecular Weight: 11kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: NM_004591.3
• Gene Symbol: CCL20/MIP-3 alpha
• Gene ID (NCBI): 6364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78556-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.