Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12363-1-AP targets CCNDBP1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3032 Product name: Recombinant human CCNDBP1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-360 aa of BC009689 Sequence: MASATAPAAAVPTLASPLEQLRHLAEELRLLLPRVRVGEAQETTEEFNREMFWRRLNEAAVTVSREATTLTIVFSQLPLPSPQETQKFCEQVHAAIKAFIAVYYLLPKDQGITLRKLVRGATLDIVDGMAQLMEVLSVTPTQSPENNDLISYNSVWVACQQMPQIPRDNKAAALLMLTKNVDFVKDAHEEMEQAVEECDPYSGLLNDTEENNSDNHNHEDDVLGFPSNQDLYWSEDDQELIIPCLALVRASKACLKKIRMLVAENGKKDQVAQLDDIVDISDEISPSVDDLALSIYPPMCHLTVRINSAKLVSVLKKALEITKASHVTPQPEDSWIPLLINAIDHCMNRIKELTQSELEL Predict reactive species |
| Full Name | cyclin D-type binding-protein 1 |
| Calculated Molecular Weight | 360 aa, 40 kDa |
| GenBank Accession Number | BC009689 |
| Gene Symbol | CCNDBP1 |
| Gene ID (NCBI) | 23582 |
| RRID | AB_2877849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95273 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCNDBP1, also named as DIP1, GCIP and HHM, is a helix-loop-helix protein which suppresses tumorigenesis. CCNDBP1 specifically interacts with one of the class III HDAC proteins, SirT6, which is important for maintaining genome stability. CCNDBP1 may negatively regulate cell cycle progression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| IHC protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| WB protocol for CCNDBP1 antibody 12363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCNDBP1 Polyclonal antibody (Cat# 12363-1-AP) has 11 citations published in journals including World J Gastroenterol, Biomed Pharmacother, Mol Med Rep, Cancers, J Cell Mol Med, Oncol Rep, Front Oncol, Cancer Manag Res, Onco Targets Ther, and Am J Transl Res. Research fields span intestinal regeneration, vascular biology, and multiple cancer types including colon, prostate, endometrial, gastric, pancreatic, melanoma, glioma, liposarcoma, and multiple myeloma.
| Species | Application | Title |
|---|---|---|
World J Gastroenterol Ubiquitin-specific protease 22 enhances intestinal cell proliferation and tissue regeneration after intestinal ischemia reperfusion injury. | ||
Biomed Pharmacother Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ. | ||
Mol Med Rep Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways. | ||
Cancers (Basel) Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways. | ||
J Cell Mol Med LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1. | ||
