Cyclin H Polyclonal antibody

Cyclin H Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10718-1-AP

Synonyms

CAK, CCNH, cyclin H, MO15 associated protein, p34, p37



Tested Applications

Positive WB detected inA431 cells, HuH-7 cells, mouse brain tissue, A375 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10718-1-AP targets Cyclin H in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag1088
Full Name cyclin H
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005280
Gene symbol CCNH
Gene ID (NCBI) 902
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cyclin H antibody 10718-1-APDownload protocol
IHC protocol for Cyclin H antibody 10718-1-APDownload protocol
IF protocol for Cyclin H antibody 10718-1-APDownload protocol
IP protocol for Cyclin H antibody 10718-1-APDownload protocol
FC protocol for Cyclin H antibody 10718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols