CCNY Monoclonal antibody

• Featured Product
• KD/KO Validated

CCNY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2C9E3

Cat no : 66865-1-Ig

Synonyms

C10orf9, CBCP1, CCNY, CFP1, Cyc Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin X, cyclin Y, FLJ95513

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Validation Data Gallery

• 

  WB analysis using 66865-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66865-1-Ig

  WB result of CCNY antibody (66865-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNY transfected A549 cells.

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  WB analysis of NIH/3T3 using 66865-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66865-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66865-1-Ig targets CCNY in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCNY fusion protein Ag12691
• Full Name: cyclin Y
• Calculated Molecular Weight: 33 kDa, 39 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC104773
• Gene Symbol: CCNY
• Gene ID (NCBI): 219771
• RRID: AB_2882202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy.; Authors - Marc Dohmen