|Positive WB detected in||mouse lung tissue, THP-1 cells, mouse liver tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13861-1-AP targets CCPG1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCPG1 fusion protein Ag4841|
|Full Name||cell cycle progression 1|
|Calculated molecular weight||87 kDa|
|Observed molecular weight||87 kDa|
|GenBank accession number||BC034914|
|Gene ID (NCBI)||9236|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CCPG1 has 5 isoforms with MW 87 kDa, 93 kDa, 71 kDa, 68 kDa and 48 kDa. This antibody is induced by C-terminal of CCPG1. It recognizes all the five isoforms.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
H (Verified Customer) (04-05-2020)
This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.