• Featured Product
  • KD/KO Validated

CCPG1 Polyclonal antibody

CCPG1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13861-1-AP

Synonyms

CCP8, CCPG1, cell cycle progression 1, CPR8, KIAA1254



Tested Applications

Positive WB detected inHepG2 cells, L02 cells, THP-1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

13861-1-AP targets CCPG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCPG1 fusion protein Ag4841
Full Name cell cycle progression 1
Calculated molecular weight 87 kDa
Observed molecular weight110 kDa
GenBank accession numberBC034914
Gene symbol CCPG1
Gene ID (NCBI) 9236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cell-cycle progression gene 1 (CCPG1) is a receptor for ER stress-induced ER-phagy. CCPG1 localizes to perinuclear ER and in small foci at the ER periphery. It contains a LIR at the N-terminal cytosolic domain engaging LC3/GABARAP. (PMID: 29802216, PMID: 29290589)

Protocols

Product Specific Protocols
WB protocol for CCPG1 antibody 13861-1-APDownload protocol
IHC protocol for CCPG1 antibody 13861-1-APDownload protocol
IF protocol for CCPG1 antibody 13861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

Authors - Heeseon An
humanWB

Mol Cell

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Authors - Haruka Chino
mouseWB,IHC

Phytomedicine

Piperine alleviates acute pancreatitis: A possible role for FAM134B and CCPG1 dependent ER-phagy

Authors - Weiguo Huang
humanWB

Biol Direct

Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins.

Authors - Maria Sol Herrera-Cruz
humanWB

Mol Biol Cell

Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin.

Authors - Andrew Kennedy
humanWB,IP

Dev Cell

CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.

Authors - Matthew D Smith
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-05-2020)

This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116
CCPG1 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:13861-1-AP)