• Featured Product
  • KD/KO Validated

CCPG1 Polyclonal antibody

CCPG1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13861-1-AP

Synonyms

CCP8, CCPG1, cell cycle progression 1, CPR8, KIAA1254



Tested Applications

Positive WB detected inmouse lung tissue, THP-1 cells, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13861-1-AP targets CCPG1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCPG1 fusion protein Ag4841
Full Name cell cycle progression 1
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC034914
Gene symbol CCPG1
Gene ID (NCBI) 9236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCPG1 has 5 isoforms with MW 87 kDa, 93 kDa, 71 kDa, 68 kDa and 48 kDa. This antibody is induced by C-terminal of CCPG1. It recognizes all the five isoforms.

Protocols

Product Specific Protocols
WB protocol for CCPG1 antibody 13861-1-APDownload protocol
IHC protocol for CCPG1 antibody 13861-1-APDownload protocol
IP protocol for CCPG1 antibody 13861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

Authors - Heeseon An
humanWB

Mol Cell

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Authors - Haruka Chino
humanWB,IP

Dev Cell

CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.

Authors - Matthew D Smith
  • KO Validated
humanWB

Autophagy

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Authors - Svenja Zielke
humanWB

Mol Cell

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

Authors - Corey N Cunningham
  • KD Validated
humanWB

Mol Cell

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Authors - Susanne Zellner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-05-2020)

This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116
CCPG1 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:13861-1-AP)