Multi-rAb® CCR2a-specific Multi-Recombinant antibody

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• Multi-rAb® CCR2a-specific Multi-Recombinant antibody (RMX00052)

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  WB analysis using RMX00052

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00052 (CCR2a-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using RMX00052

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00052 (CCR2a-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using RMX00052

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00052 (CCR2a-specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using RMX00052

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CCR2a-specific antibody (RMX00052) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCR2a-specific Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00052

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2

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Tested Applications

• Positive WB detected in: A431 cells, mouse skeletal muscle tissue, HEK-293 cells, rat skeletal muscle tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00052 targets CCR2a-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chemokine (C-C motif) receptor 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 37-42 kDa
• GenBank Accession Number: NM_000647
• Gene Symbol: CCR2
• Gene ID (NCBI): 729230
• RRID: AB_3750104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P41597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.