CCT2 Polyclonal antibody, PBS Only

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• CCT2 Polyclonal antibody, PBS Only (24896-1-PBS)

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  WB analysis of mouse testis using 24896-1-AP (same clone as 24896-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 24896-1-AP (same clone as 24896-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 24896-1-AP (same clone as 24896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24896-1-AP (CCT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CCT2 antibody (24896-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CCT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24896-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

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Product Information

24896-1-PBS targets CCT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19264

  Product name: Recombinant human CCT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 186-535 aa of BC113516

  Sequence: AVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

• Full Name: chaperonin containing TCP1, subunit 2 (beta)
• Calculated Molecular Weight: 535 aa, 57 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC113516
• Gene Symbol: CCT2
• Gene ID (NCBI): 10576
• RRID: AB_2879783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78371
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)