CCT3 Polyclonal antibody

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CCT3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10571-1-AP Copied!

Publications (20)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TCP 1 gamma, CCT gamma, CCTG, CCTgamma, CCT-gamma

• 

  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 10571-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 10571-1-AP

  IP result of anti-CCT3 (IP:10571-1-AP, 4ug; Detection:10571-1-AP 1:15000) with mouse brain tissue lysate 1720 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 10571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10571-1-AP (CCT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10571-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10571-1-AP

  Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 10571-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat kidney tissue, HeLa cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, U2OS cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10571-1-AP targets CCT3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0843

  Product name: Recombinant human CCT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 246-545 aa of BC006501

  Sequence: EYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE

• Full Name: chaperonin containing TCP1, subunit 3 (gamma)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC006501
• Gene Symbol: CCT3
• Gene ID (NCBI): 7203
• RRID: AB_2073658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49368
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT3 protein (full name: chaperonin containing TCP1 subunit 3, also known as the gamma subunit of TCP-1 protein) is an important member of the TCP-1 chaperonin family. As one of the eight essential subunits of the CCT/TRiC complex, its core function is to act as a molecular chaperone that assists in the correct folding of other cellular proteins under ATP hydrolysis. It is particularly involved in the folding process of key cytoskeletal components such as actin and tubulin, while also participating in important biological activities including ciliogenesis and telomere maintenance.

Publications

What published studies show

The anti-CCT3 antibody (Cat# 10571-1-AP) has 20 citations published in journals including Nature, Cell, Molecular Cell, PNAS, Nature Genetics, Signal Transduction and Targeted Therapy, Science Translational Medicine, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Death & Disease, Journal of Cell Biology, and others. Research fields span chaperonin function, cilia/flagella biology, protein homeostasis, liver disease, ferroptosis, cancer (hepatocellular, lung, pancreatic, bladder), muscle aging, spermatogenesis, and cardiac protection.

Automatically generated from the 20 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Signal Transduct Target Ther; Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease; Authors - Boyuan Ma
human	WB,CoIP	Nature; In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor; Authors - Huaipeng Xing
mouse	WB	Cell; Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.; Authors - Erik McShane
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
human	CoIP	Mol Cell; FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways; Authors - Min Huang
mouse	WB,IP,IHC,IF	Mol Cell; PDCD5 promotes substrate release from the TRiC complex in cilia and flagella.; Authors - Huafang Wei