CCT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCT3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10571-1-AP

Synonyms

TCP 1 gamma, hTRiC5, Chaperonin containing T-complex polypeptide 1 subunit 3, CCT-gamma, CCTgamma

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Validation Data Gallery

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  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10571-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10571-1-AP

  IP result of anti-CCT3 (IP:10571-1-AP, 3ug; Detection:10571-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human liver cancer using 10571-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10571-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10571-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 10571-1-AP

  Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 10571-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat kidney tissue, HeLa cells, Jurkat cells, U2OS cells, mouse brain tissue, rat brain tissue, mouse kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10571-1-AP targets CCT3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCT3 fusion protein Ag0843
• Full Name: chaperonin containing TCP1, subunit 3 (gamma)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC006501
• Gene Symbol: CCT3
• Gene ID (NCBI): 7203
• RRID: AB_2073658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
mouse	WB	Cell; Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.; Authors - Erik McShane
human,mouse	WB	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S ; KD Validated
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human	WB,IF	Dev Biol; Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae.; Authors - Redgrove Kate A KA
human	WB	Toxicol Appl Pharmacol; Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1.; Authors - Hongyang Shi ; KD Validated