CCT6A-Specific Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CCT6A-Specific Polyclonal antibody (19793-1-AP)

• Featured Product
• KD/KO Validated

CCT6A-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19793-1-AP Copied!

Publications (15)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CCT6A, Acute morphine dependence-related protein 2, CCT zeta, CCT zeta 1, CCT6

• 

  WB analysis using 19793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 19793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 19793-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 19793-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 19793-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CCT6A-Specific antibody (19793-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CCT6A-Specific in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: A431 cells, A549 cells. HeLa cells, mouse liver tissue, EC109 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19793-1-AP targets CCT6A-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chaperonin containing TCP1, subunit 6A (zeta 1)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_001762
• Gene Symbol: CCT6A
• Gene ID (NCBI): 908
• RRID: AB_10638922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P40227
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT6A, also named as CCT6, CCTZ, CCT-zeta,Tcp20 and HTR3, belongs to the TCP-1 chaperonin family. CCT6A is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. CCT6A is known to play a role, in vitro, in the folding of actin and tubulin. The antibody has no cross reaction to CCT6B.

Publications

What published studies show

Anti-CCT6A (Catalog No. 19793-1-AP) has 15 total citations published in journals including Nature, Cell, Molecular Cell, Journal of Clinical Investigation, Advanced Science, Signal Transduction and Targeted Therapy, Cancer Letters, and Molecular Carcinogenesis. Research fields span chaperonin/TRiC complex biology, aggrephagy, nonalcoholic fatty liver disease, multiple sclerosis, exosome biology, and multiple cancer types including gastric, pancreatic, colorectal, lung, liver, colon, and osteosarcoma.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Signal Transduct Target Ther; Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease; Authors - Boyuan Ma
human	WB	Nature; In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor; Authors - Huaipeng Xing
human	WB,IF	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
mouse	WB,IP,IHC,IF	Mol Cell; PDCD5 promotes substrate release from the TRiC complex in cilia and flagella.; Authors - Huafang Wei
human	IF	J Clin Invest; ANKRD55 is a key regulator of T cell inflammation in multiple sclerosis; Authors - Chuyu Wu
human	WB,IP	Adv Sci (Weinh); Exosomal CCT6A Secreted by Cancer-Associated Fibroblasts Interacts with β-Catenin to Enhance Chemoresistance and Tumorigenesis in Gastric Cancer; Authors - Hui Sun ; KD Validated