MME,CD10 Polyclonal antibody
MME,CD10 Polyclonal Antibody for FC, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 23782-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human kidney tissue |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
23782-1-AP targets MME,CD10 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MME,CD10 fusion protein Ag20634 |
Full Name | membrane metallo-endopeptidase |
Calculated molecular weight | 750 aa, 86 kDa |
GenBank accession number | BC101632 |
Gene symbol | MME |
Gene ID (NCBI) | 4311 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
Protocols
Product Specific Protocols | |
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IHC protocol for MME,CD10 antibody 23782-1-AP | Download protocol |
IF protocol for MME,CD10 antibody 23782-1-AP | Download protocol |
FC protocol for MME,CD10 antibody 23782-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |