Neprilysin/CD10 Polyclonal antibody

• 

  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Neprilysin/CD10 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 23782-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF-P, ELISA

Synonyms

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

---

Tested Applications

• Positive IHC detected in: human kidney tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

23782-1-AP targets Neprilysin/CD10 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neprilysin/CD10 fusion protein Ag20634
• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• GenBank Accession Number: BC101632
• Gene Symbol: CD10
• Gene ID (NCBI): 4311
• RRID: AB_2879323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08473
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Publications

Species	Application	Title
mouse	IF	Clin Breast Cancer; Inhibitory Impact Of Cinobufagin In Triple-Negative Breast Cancer Metastasis: Involvements Of Macrophage Reprogramming Through Upregulated MME and Inactivated FAK/STAT3 Signaling; Authors - Zhaohui Zhu