FcZero-rAb® PE Anti-Human CD100 Rabbit Recombinant Antibody

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• FcZero-rAb® PE Anti-Human CD100 Rabbit Recombinant Antibody (PE-FcA98345-2)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD100 FcZero-rAb^® Recombinant Antibody for FC

Cat No. PE-FcA98345-2 Copied!

Clone No. 242691F8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SEMA4D, A8, CD 100, CD100 antigen, SEM4D

• 

  FC experiment of human PBMCs using PE-FcA98345-2

  1x10^6 human PBMCs were surface stained with FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD19, and 5 ul PE Anti-Human CD100 Rabbit RecAb (PE-FcA98345-2, Clone: 242691F8) or PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using PE-FcA98345-2

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD100 Rabbit RecAb (PE-FcA98345-2, Clone: 242691F8) (red) or PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98345-2 targets CD100 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Calculated Molecular Weight: 96 kDa
• GenBank Accession Number: BC054500
• Gene Symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• RRID: AB_3749880
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92854
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.