Anti-Mouse CD107a / LAMP1 (1D4B)
CD107a / LAMP1 Monoclonal Antibody for FC, IF
Host / Isotype
Rat / IgG2a
Reactivity
Mouse and More (1)
Applications
IF, FC
Conjugate
Unconjugated
CloneNo.
1D4B
Cat no : 65050-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | NIH/3T3 cells |
Positive FC detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 1 publications below |
Product Information
65050-1-Ig targets CD107a / LAMP1 in IF, FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Cited Reactivity | mouse |
Host / Isotype | Rat / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Plasma membrane fraction of mouse embryo NIH/3T3 cell line |
Full Name | lysosomal-associated membrane protein 1 |
GenBank accession number | BC006785 |
Gene symbol | Lamp1 |
Gene ID (NCBI) | 16783 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | Borate buffered saline, pH 8.2. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CD107a / LAMP1 antibody 65050-1-Ig | Download protocol |
FC protocol for CD107a / LAMP1 antibody 65050-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Physiol Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes |