Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells, C2C12 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in 100 ul suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65050-1-Ig targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Plasma membrane fraction of mouse embryo NIH/3T3 cell line Predict reactive species |
| Full Name | lysosomal-associated membrane protein 1 |
| GenBank Accession Number | BC006785 |
| Gene Symbol | CD107a |
| Gene ID (NCBI) | 16783 |
| RRID | AB_2881466 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P11438 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD107a / LAMP1 antibody 65050-1-Ig | Download protocol |
| IF protocol for CD107a / LAMP1 antibody 65050-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The rat anti-mouse CD107a/LAMP1 monoclonal antibody 65050-1-Ig (1D4B) has 18 total citations.
Journals: Bioactive Materials, Molecular Neurodegeneration, Nature Communications, Journal of Advanced Research, Journal of Controlled Release, Acta Pharmacologica Sinica, NPJ Parkinson's Disease, Drug Delivery, Ecotoxicology and Environmental Safety, Inflammation, Scientific Reports, Journal of Cellular Physiology, Journal of Leukocyte Biology, Cell Structure and Function, bioRxiv, and Research Square.
Research fields: Lysosomal biology/autophagy, innate immune signaling (TLR3, cGAS-STING), neuroinflammation and neurodegeneration (Parkinson's, Alzheimer's), stroke, cancer immunology and therapy, colorectal/glioblastoma oncology, fibrosis, pancreatitis, and drug delivery. Nearly all citations use it in immunofluorescence (IF) with mouse samples.
| Species | Application | Title |
|---|---|---|
Bioact Mater Biomimetic stress granules replenish lysosomal repair to reinstate macrophage immunometabolic antibacterial programs. | ||
Mol Neurodegener Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency | ||
Nat Commun Ubiquitination by HRD1 is essential for TLR3 trafficking and its innate immune signaling. | ||
J Adv Res Parkin modulates the hepatocellular carcinoma microenvironment by regulating PD-1/PD-L1 signalling | ||
J Control Release An oral vaccine based on stable peptide-chitosan conjugate targeting DEC-205 for cancer immunotherapy. | ||
Acta Pharmacol Sin Deficient chaperone-mediated autophagy drives multiorgan fibrogenesis via SMAD2/4 stabilization to sustain TGFβ-SMAD signaling. |
Reviews
What customers say
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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (06-19-2026) | good for WB
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