CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (1D4B)

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (2)

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Publications

2

CloneNo.

1D4B

Cat no : CL647-65050

Synonyms

AI196048, CD107a, Lamp 1, Lamp1

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL647-65050

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 647 CD107a antibody (CL647-65050, Clone: 1D4B ) at dilution of 1:400.

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  FC experiment of NIH/3T3 using CL647-65050

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (CL647-65050, Clone:1D4B) (red), or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL647-65050 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: Lamp1
• Gene ID (NCBI): 16783
• RRID: AB_2883653
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publications

Species	Application	Title
mouse	IF	Free Radic Biol Med; STING promotes ferroptosis through NCOA4-dependent ferritinophagy in acute kidney injury; Authors - Lini Jin
human	IF	J Zhejiang Univ Sci B; Fibroblast growth factor 21 (FGF21) attenuates tacrolimus-induced hepatic lipid accumulation through transcription factor EB (TFEB)-regulated lipophagy; Authors - Zhensheng Zhang

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C