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CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (1D4B)

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D4B

Cat no : CL647-65050

Synonyms

AI196048, CD107a, Lamp 1, Lamp1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (1D4B (CL647-65050)

    IF Staining of NIH/3T3 using CL647-65050

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 647 CD107a antibody (CL647-65050, Clone: 1D4B ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (1D4B (CL647-65050)

    FC experiment of NIH/3T3 using CL647-65050

    1X10^6 NIH/3T3 cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD107a (CL647-65050, Clone:1D4B) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65050 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Plasma membrane fraction of mouse embryo NIH/3T3 cell line
Full Name lysosomal-associated membrane protein 1
GenBank accession numberBC006785
Gene symbol Lamp1
Gene ID (NCBI) 16783
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65050Download protocol
Standard Protocols
Click here to view our Standard Protocols