CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (1D4B)
CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1D4B
Cat no : CL647-65050
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | NIH/3T3 cells |
Positive FC detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Product Information
CL647-65050 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Plasma membrane fraction of mouse embryo NIH/3T3 cell line |
Full Name | lysosomal-associated membrane protein 1 |
GenBank accession number | BC006785 |
Gene symbol | Lamp1 |
Gene ID (NCBI) | 16783 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65050 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |