Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-65050 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Plasma membrane fraction of mouse embryo NIH/3T3 cell line Predict reactive species |
| Full Name | lysosomal-associated membrane protein 1 |
| GenBank Accession Number | BC006785 |
| Gene Symbol | CD107a |
| Gene ID (NCBI) | 16783 |
| RRID | AB_2882955 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P11438 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD107a / LAMP1 antibody APC-65050 | Download protocol |
| IF protocol for APC CD107a / LAMP1 antibody APC-65050 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
APC-65050 is the Anti-Mouse CD107a/LAMP1 (1D4B) monoclonal antibody. It has 2 citations. One appears in Nature Communications (2025), studying cascaded immunotherapy using implantable dual-drug depots releasing STING agonists and apoptosis inducers. The other is in Pharmacological Research (2023), investigating how RHBDF1 deficiency suppresses melanoma glycolysis and enhances immunotherapy efficacy. Both studies used the antibody for immunofluorescence in mouse models, spanning the research fields of cancer immunotherapy and tumor metabolism.
| Species | Application | Title |
|---|---|---|
Nat Commun Cascaded immunotherapy with implantable dual-drug depots sequentially releasing STING agonists and apoptosis inducers | ||
Pharmacol Res RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation via TRIM32 |
