APC Anti-Mouse CD107a (1D4B)

CD107a Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

IF, FC

Conjugate

APC Fluorescent Dye

CloneNo.

1D4B

Cat no : APC-65050

Synonyms

AI196048, CD107a, Lamp 1, Lamp1



Tested Applications

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

APC-65050 targets CD107a in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen Plasma membrane fraction of mouse embryo NIH/3T3 cell line
Full Name lysosomal-associated membrane protein 1
GenBank accession numberBC006785
Gene symbol Lamp1
Gene ID (NCBI) 16783
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and a stabilizer.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
IF protocol for APC CD107a antibody APC-65050Download protocol
FC protocol for APC CD107a antibody APC-65050Download protocol
Standard Protocols
Click here to view our Standard Protocols