CoraLite®594 Anti-Mouse CD107a / LAMP1 (1D4B)

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  IF Staining of NIH/3T3 using CL594-65050

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite®594 CD107a antibody (CL594-65050, Clone: 1D4B ) at dilution of 1:100.

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  FC experiment of NIH/3T3 using CL594-65050

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse CD107a / LAMP1 (1D4B) (CL594-65050, Clone:1D4B) (red) or 0.25 ug CoraLite®594 Rat IgG2a Isotype Control (2A3) (CL594-65209, Clone: 2A3) (blue) . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-65050

Clone No.1D4B

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CD107a, Lamp1, 1D4B, Lamp 1

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65050 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Plasma membrane fraction of mouse embryo NIH/3T3 cell line

• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• RRID: AB_2919924
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P11438
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).