CoraLite® Plus 647 Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG1 Recombinant Antibody

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  FC experiment of HeLa using CL647-65519

  1x10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107a Mouse Recombinant Antibody (CL647-65519, Clone:H4A3) or Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using CL647-65519

  1x10^6 HeLa cells were intracellularly stained with 5 ul Anti-Human CD107a Mouse Recombinant Antibody (CL647-65519, Clone:H4A3) or Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using CL647-65519

  1x10^6 HeLa cells were intracellularly stained with 5 ul Anti-Human CD107a Mouse Recombinant Antibody (CL647-65519, Clone:H4A3) or 5 ul Anti-Human CD107a Mouse Monoclonal Antibody (CL647-65051, Clone:H4A3). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CD107a / LAMP1 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-65519

Clone No.H4A3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, H4A3

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65519 targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human adherent peripheral blood cells

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC006345
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• RRID: AB_3673595
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P11279
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).