FcZero-rAb® Biotin Anti-Human CD107a / LAMP1 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human CD107a / LAMP1 Rabbit Recombinant Antibody (Biotin-FcA98266-2)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD107a / LAMP1 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98266-2

Clone No.242116E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1

• 

  FC experiment of Jurkat using Biotin-FcA98266-2

  1x10^6 Jurkat cells were surface stained with 0.25 ug Biotin Anti-Human CD107a / LAMP1 Rabbit RecAb (Biotin-FcA98266-2, Clone:242116E8) (red), or 5 ul FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9)(blue) and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: Jurkat cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98266-2 targets CD107a / LAMP1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2611

  Product name: Recombinant Human CD107a/LAMP1 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 26-381 aa of NM_005561.4

  Sequence: ASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENS

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: NM_005561.4
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• RRID: AB_3747007
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11279-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).